STED Fig. 1: Comparison of different virus particle sizes with the resolution of different imaging techniques.

Super resolution microscopy is a widely used research method for studying biological features beyond the diffraction limit, such as nuclear pores, clathrin coated vesicles, the periodicity of the actin skeleton in neurons, and viruses. In this white paper we will focus on the advantages of STED microscopy and its contributions to the virology field. We will provide insight into the advances in virus research made possible by STED microscopy and give a hint to where the journey might go.