Tomocube’s holotomography microscopy series utilize optical diffraction tomography (ODT), which enable users to quantitatively and noninvasively investigate biological cells and thin tissues.
ODT reconstructs the 3D refractive index (RI) distribution of live cells and by doing so, provides structural and chemical information about the cell, including dry mass, morphology, and dynamics of the cellular membrane. With holotomography, it makes the structure of living cells three dimensionally without any labelling. In the case of conventional cell imaging equipment, fluorescence staining should be used mainly to acquire three-dimensional images. It can be done after acquiring many flat images in the Z-axis direction through a confocal method. On the other hand, this microscope can acquire 3D image quickly and easily by applying a tomography algorithm after measuring 2D holographic images. This technology can reconstruct 3D structures by using 3D refractive index information in the cell, and the refractive index information can provide quantitative information because it is an intrinsic density information in a cell. The resolution of the image through tomography reconstruction process can be about twice as high as that of general optical equipment with fluorescence imaging, the information about molecular specificity can be provided to the holotomographic image.
- 3D correlative microscopy of holotomography and fluorescence can be achieved in one microscope.
- Holotomographic image enables quantitative analysis of volume, surface area, concentration, dry mass, etc. in the live cell.
- Three channels of fluorescence image can provide information about molecular specificity.
- Heterogenous time-lapse imaging of holotomography and fluorescence enables the monitoring of cells and their structures with minimal stress.
- 2D phase imaging and computerized BF, phase contrast, DIC imaging is possible.
- Label-free holotomographic image is built up (or constructed) with RI distribution in the cell.
- High resolution holotomographic image up to 110nm in lateral, 356nm in axial
- Fast 3D tomography image acquisition up to 2.5 frame per second
- Very low phototocixity suitable for live cell imaging
- Free digital coloring on specific RI for 3D/4D visualization capability
- Free and fast processing without slowing image acquisition
- No mechanical movement during image acquisition